UPR Medical Sciences Campus
Dr. Martine Behra’s lab consists of 950 sq ft of temporary laboratory space on the 5th floor of the campus Main Building (Dept. Anatomy & Neurobiology [A&N]) that is immediately available for this project, while the two permanent research labs that have been assigned on this same floor are being renovated. Each of these permanent labs is ~500 sq ft; one will house the zebrafish room with the needed racks and filtration/pumping equipment, a preparatory area for working with fish embryos, larvae and adults, and a microscope room, equipped with a Zeiss AXIO OBSERVER Z1 inverted scope with Vivatome System. The second lab will house the PI's office, bench areas, freezers, refrigerators, storage cabinets and student/postdoc desk/study area. The renovation of these labs is expected to be finished by January 2011. The temporary lab space has ample bench areas and storage cabinets, and space for microscopes, freezers, refrigerators and desks. One area has been designated to house temporary fish racks that will be provided by the company that is manufacturing the equipment of the permanent fish room.
Animal: Zebrafish will be housed in specialized racks containing tanks and a self-contained water filtration/pumping system in a room devoted to this purpose. The specialized fish racks are part of a ZebTEC Aquatic Housing System, which includes ten 54" painted 316L S/S multilinking 6-row racks, each complete with reservoir, prefilter, and double biofilter, and including 15 (1.1L) tanks + 50 (3.5 L) tanks, with a vertical drain position. This system also provides the following key features: siphon system at tank level, automated water quality dosing equipment for pH and conductivity standard, redundant heating system and temperature control, touch screen controller with complete system control,password protected parameter settings, automated maintenance reminders and alarms, automated hourly water change providing more stable water quality, and 24 hour monitoring of up to 17 different maintenance and safety parameters. The room is also equipped with a 160 gallon/day Reverse Osmosis (RO) filtration unit with 110 gallon storage tank. A separate backup 44 gallon/day RO filtration unit with a 55 gallon storage tank is also available at a nearby room. The fish room (Rm A505, 350 sq ft) will be considered as a satellite of the campus main AALAC-accredited Animal Care Facility (ACF) and will be serviced and monitored daily by a technician hired for that purpose. The ACF also has veterinarians available on site for regular care and consultation.
UPR Medical Sciences Campus Core Facilities
Proteomics Core: HPLC, fast protein liquid chromatography (FPLC), 1 and 2D gel electrophoresis, 2D-DIGE, SELDI-TOF ProteinChip Biomarker System, Biomek 2000 Protein Chip Arrays System Integration Package, LTQ XL tandem mass spectrometer.
Molecular Biology Core: BioRad iCycler iQ Real-Time Detection System and Applied Biosystems Bioanalyzer.
Functional Genomics Research Center: Acturus PixCel II system for Laser Capture Microdissection, NanoDrop Spectrophotometer, 2100 Bioanalyzer, Agilent DNA Microarray System;
Microbiology Core: BD FACSAria cell sorter/flow cytometer.