UPR Río Piedras
The College of Natural Sciences has a total of 34 teaching labs for use by six departments (Biology, Chemistry, Computer Science, Environmental Sciences, Mathematics, and Physics) and 18,000 sq.ft. of shared space in the new UPRRP Molecular Sciences Building located approximately 1 mile from the UPRRP campus. The Chemistry labs were renovated in 2010. Three auditoriums were upgraded with videoconferencing equipment in 2009. The Information and Technology Center contains 22 computer workstations with software for remote sensing, GIS, word processing and presentation, statistical analysis and others. The High Performance Computing facility (HPCF) offers super computing capacity, a bioinformatics resource center, and videoconferencing capacity. UPRRP is part of Internet2. Our new node for transcriptomics is housed at the HPCF and provides an Intel Xeon E5-4620 Sandy Bridge 2.2 GHz Eight Core 32nm CPU with 1 TB of memory and 1 TB or storage that is currently being upgraded to 25 TB.
Environmental Sciences has a total of four teaching labs, including a GIS computer lab, a geophysics lab, a land use lab, and an environmental sciences lab equipped mainly for water quality analysis (turbidometers, colorimeters, UV/Vis spectrophotometers, incubators, DO meters, pH meters, and a variety of test kits for nutrient and fecal coliform contamination. Dr. Loretta Roberson has a lab (900 sq.ft.) with a variety of equipment necessary to measure algal and coral physiology and growth including two 100-gallon tank systems and numerous 20-gallon aquaria in a new aquaculture facility, an underwater pulse-amplitude modulated fluorometer (Diving PAM), dual chamber Rank Brothers oxygen electrode system, portable dissolved oxygen sensors, underwater light sensors and loggers, portable turbidometers and colorimeters, analytical balances, and an underwater camera for rapid surveys. In addition we have analytical equipment for water quality analysis including a SmartChem Discrete Analyzer, CHNSO Analyzer, GC-MS, accelerated solvent extractor, UV/VIS spectrometers. We share space in the Instrumental Analysis laboratory (1,500 sq ft) of the Department of Chemistry to house an ICP-MS and Atomic Absorption Spectrometer.
The Chemistry Department laboratories include two 3,750 sq.ft. laboratories for Analytical Chemistry, one Instrumental Analysis laboratory (1,500 sq ft) and lab spaces. The instrumental analysis lab is shared with environmental sciences and is equipped for biofuel and environmental contaminant studies and includes an Inductively Coupled Plasma Mass Spectrometer, GC-MS, GC-FID, UV/VIS spectrometers, FT-IR Spectrometer, Ion Exchange Chromatograph, Atomic Absorption Spectrometer, and a Potentiostat, directed by Dr. Liz Diaz. In addition, each laboratory is equipped with MeasureNet systems that enable users to acquire data using different probes connected to the network (pressure, pH, temperature, volume, dissolved oxygen, voltage, colorimeters, HPLC and GC with thermocouple detector) as well as share a network-connected UV/Vis spectrophotometer and a multi-function colorimeter.
The Biology Department has a state-of-the-art Sequencing and Genotyping Facility and a Bioinformatics Satellite Laboratory well equipped with relevant software, an extensive herbarium, and an invertebrate collection. For field support the department maintains a 25' boat equipped with two 125HP outboard engines, a sonar, and a Garmin 215 GPS. This boat is kept at a marina in Fajardo on the east coast of Puerto Rico. Dr. Alberto Sabat also has 1,500 square feet of laboratory and office space in the Natural Sciences Building of the Río Piedras Campus, with space for student offices, a wet table to keep aquaria, and computers to perform data analysis and preparation of manuscripts and reports.
The Eduardo Rosa-Molinar Laboratory focuses on circuit neuroscience. Dr. Rosa-Molinar has 1,698 sq. ft. of laboratory space. The main laboratory has two 6 ft. chemical fume hoods, 4 refrigerators, 1 -20° explosion-proof freezer, two double tub sinks, and various types of water (chlorinated tapwater, distilled, PCR grade, or RO water). The laboratory has sufficient bench space, is fitted with safety equipment including an eye wash station, fire extinguishers, fire blanket, first aid kit, respirators, safety face mask shields, a CBG Biotech formaldehyde recycling unit, CBG Biotech Solvent Recycler and hazardous chemical storage (acid, base, and flammables) cabinets and chemical containment cabinets. The laboratory has sufficient bench and desk space to accommodate 5 people comfortably.
The fish culture room houses one Isoplex stand-alone and one Isoplex bench-top recirculating units with biological and chemical filtration. The stand-alone unit is composed of four shelves, each shelf holding twelve 2.75-liter polycarbonate tanks, and the bench-top unit is composed of two shelves, each shelf holding sixteen 1-liter polycarbonate tanks. All racks are made from 316L-grade stainless steel. Each tank is self-cleaning. The water within the unit is ultraviolet sterilized (dosage of 60,000 Microwatts/sec/cm2 ). 25 % water changes are made automatically twice a week. The Isoplex units are connected to a reverse osmosis unit from Millipore. Water quality data (pH, temperature, conductivity and dissolved oxygen) is collected and the water is conditioned using marine salts and sodium bicarbonate through an automated YSI 5200 water quality monitoring and dosing system.
The main laboratory contains a Dell Inspiron Desktop 530 with 4GB of RAM, 250GB SATA hard drive, DVD writer, with a 21” super VGA monitor, SGI O2 with a 270 MHz processor with 8 GB of RAM, 48 GB hard drive, readable and/or writeable CD drive, with dual 21” super VGA monitors. A Dell Dimension 8100 1.4 GHZ Pentium 4 Processor 1G RAM 180 GB hard drive equipped with drivers for a digital 35-mm film recorder, 35-mm slide scanner and transparency adapter. A Power Edge 3000 Pentium IV processor with 1GB RAM, dual 18 GB hard drives RAID server. The microscopy suite has a Dell XPS Dimension Gen 2 with 4GB of RAM, RAID 0 120GB SATA hard drives, 256MB Radeon 360 video card, DVD writer, with 24" Widescreen Analog/Digital LCD Monitor and a Praetorian with 2GB of RAM, two 300GB SATA hard drives, 256MB Radeon X1555 video card, DVD writer, with a 20" Widescreen LCD Monitor for the Nikon C1 confocal module control. A Dell Optiplex with Pentium 4 processor with 512 MB RAM, 40 GB hard drive. A Dell XPS Laptop M1730 with 4GB of RAM, 300GB SATA hard drive, DVD writer, 1024MB video card, with a 17” LCD display, Dell XPS Laptop M1710 with 4GB of RAM, 100GB SATA hard drive, DVD writer, 512MB video card, with a 17” LCD display, Dell Latitude Laptop D830 with 2GB of RAM, 120GB SATA hard drive, DVD writer, 256MB video card, with a 15.4” LCD display, and a Dell Inspiron 8500 laptop with 1.5GB RAM, 60GB Hard Drive, DVD-RW/CD-RW. All of the computers are connected to an HP 2605dn color laser printer located in the main laboratory. The main laboratory has an internal 54G wireless and 10/100 Mbps Ethernet network. The P.I. has full access to other computer facilities within the department and university, such as the High Performance Computing Facility.
Office space for Dr. Rosa-Molinar and members of his laboratory is provided by the Department of Biology and the Julio Garcia Diaz Center for Investigations in Biology (JGD). The P.I.’s office is located within the main laboratory. It contains book shelves for a small library, telephones, a FAX machine, and a vault for the storage of controlled substances. The departmental office within JGD, is located on the same hallway as the above mentioned facilities. It contains a Xerox machine, FAX, typewriters and provides secretarial services to its research faculty members.
Dr. Rosa-Molinar’s laboratory has a Lipshaw cryostat, AO sliding microtome, Vibratome 1000 Plus sectioning system, LKB Huxley ultratome, Sorvall MT5000 ultramicrotome, Sorvall MT2-B ultramicrotome, and AO rotary microtome, 6 Nikon stereomicroscope each with its own fibrooptic light sources, 2 picospritzer microinjection system, 1 BioRad Helios System, 2 Sony video cameras, 2 color monitors, 4 Qimaging Retiga Exi high sensitive digital cameras, 1 Hamamatsu Orca ER digital camera, 1 Spot RT digital camera, 1 Nikon E800 multidimensional microscope system with an Optigrid Structured Illumination module and a Nikon C1 confocal module equipped with argon and nitrogen lasers, 1Twin Epifluorescence Uncager interfaced to a Nikon E600 FN microscope equipped with argon and nitrogen lasers, 1 Nikon 80i research compound microscope equipped with 1 color digital camera, 1 B/W digital camera, and a Leica MZ16FA stereo fluorescence microscope, and FEI Nova 600 Nanolab Focused Ion Beam Scanning Electron Microscope (FIB/SEM).
The UPRRP El Verde Field Station, Directed by Dr. Alonso Ramirez, is located 23 miles from UPRRP and includes living space for up to 28 scientists, an on-site library; comprehensive collections of local fungi, ferns, dicotyledons, myriapods, arachnids, insects, amphibians, and reptiles; an NADP wet deposition station and several weather stations with digital logging and signal relay; sample preparation and nutrient analysis laboratories including sample drying, grinding, and extraction equipment, electronic balances and microbalances; a field laboratory with light meters, balances, microscopes, fiberoptic light sources, and pH meters; numerous field study sites; outdoor greenhouses; and a slow sand filter pilot plant.